Below are some frequently asked questions about our Gene Synthesis services. Should you have any questions other than listed below, feel free to contact us at email@example.com and we will be happy to clear your thoughts.
Generally, the deliverables for your synthetic gene of interest will consist of the following:
You will receive weekly email updates outlining the status of each gene you have requested us to complete.
BaseGene standard cloning vectors include pUC57-Amp, pUC57-Kan, pUC-SP and pBluescript . In general, synthetic genes will be cloned via Blunt of the standard vector, or any cloning sites you desired.
Yes, BaseGene can subclone the synthetic gene into the vector of your choice at an affordable additional cost. The vector (commercial vector or non-commercial vector) must be provided to us with a full-length sequence and other requested info.
Yes. The sequences of genes synthesized by us are confirmed by DNA sequencing, and 100% guaranteed.
Generally, it is more difficult to synthesize sequences that have:
i) GC content > 70% or < 40% in regions of at least 30 base pairs or the whole sequence (generally difficult to synthesize sequences that have either too high or low GC content; standard sequences typically have a GC content of 50%).
ii) Long sequence repeats (>12 bp) that exist in a high frequency (interval between repeats < 100bp), e.g. internal repeats, direct repeats or inverted repeats.
iii) Homopolymers longer than 15 bp.
iv) Sequences that have a high similarity with toxic genes.